Magic™ IgM 抗体纯化试剂

蛋白技术服务

抗体技术服务

细胞技术服务

核酸技术服务

基础检测服务

产品展示

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IgM单克隆抗体在治疗和诊断领域都发挥越来越重要的作用。 Protein A和Protein G亲和纯化虽然在IgG纯化上发挥着不可替代的作用,但是却不适用于纯化IgM抗体。

Creative Diagnostics提供了一种新型树脂,在纯化许多物种的IgM产品(包括人和小鼠)方面表现出色。该产品用于人IgM纯化可以达到90%的吸附率和85%的产率。除了提高产量和纯度水平外,Magic™ 人 IgM纯化树脂还解决了许多标准的亲和配体相关的缺陷,包括其他纯化方法常见的蛋白水解问题。

产品有纯化试剂盒的形式和单独的填料形式,也有1 mL和5 mL纯化柱的形式。

订购信息:
产品编号 产品名 规格 说明书
CDN-RE1 Magic™ Human IgM Purification Resin 5 mL
CDN-RE2 Magic™ Mouse IgM Purification Resin 5 mL
CDN-HPC Magic™ Human IgM Purification Column 1 mL/5 mL
CDN-MMC Magic™ Mouse IgM Purification Column 1 mL/5 mL
CDN-HMK1 Magic™ Human IgM Purification Kit 10 spin columns kit
CDN-MMK2 Magic™ Mouse IgM Purification Kit 10 spin columns kit
产品优势:
  • 高亲和力和纯度——IgM 亲和力大于5 mg/mL resin。
  • 易用性——纯化试剂盒包含所有实验所需缓冲液和试剂。
  • 可重复使用——柱子可以再生,重复使用至少10次不会降低亲和力。
  • 温和的反应条件——非变性的缓冲液条件,最大程度保证抗体的功能和稳定性。
产品数据:

Figure 1: Statistical analysis of all three human IgM purification resins in terms of % adsorption, % yield, and binding capacity; averages were based on calculations from each resin being tested in triplicate. (a) Bars represent the average percentages (±95% confidence interval) of human IgM adsorption and yield for each purification resin type. (b) Bars represent the average static binding capacity for human IgM (±95% confidence interval) for each purification resin type.

Figure 2: Coomassie-stained SDS-PAGE in reducing conditions; equivalent volumes of the flowthrough and elute fractions were analyzed. 1.7 µg of human IgM standard was loaded in the gel. The sample load was run at equivalent volume to the flowthrough fractions. Arrows indicate the position of heavy-chain (68 kDa) and light-chain (26 kDa) human IgM.

Figure 3: Analytical SEC-HPLC profiles of flowthrough and elute fractions; column: MabPac SEC-1 (30 cm); mobile phase: PBS; flow rate: 0.15 mL/min; wavelength: 280 nm; injection: 10 µL. (a) Flowthrough fraction analysis, including the sample load (starting material) and the human IgM reference standard (2.2 µg); (b) elution fraction analysis, including the human IgM reference standard (2.2 µg).